Supplementary Information r6 - albert
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چکیده
Growth and media Liquid cultures were grown at 37°C with aeration in minimal A medium (Miller, 1992) with 0.2% glycerol or glucose as the carbon source or in LB Miller medium (Difco). Colonies were grown at 37°C on 1.5% agar plates made from LB Miller medium or minimal A medium with glycerol as the carbon source. Plasmids were maintained by supplementing media with 50 μg/mL of ampicillin, 15 μg/mL of chloramphenicol or both. Experiments were performed in triplicate unless otherwise noted. For expression from IPTG-regulated promoters, basal expression was used if addition of IPTG is not mentioned explicitly.
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Regulation of Hippocampal cGMP Levels as a Candidate to Treat Cognitive Deficits in Huntington’s Disease
Huntington's disease (HD) patients and mouse models show learning and memory impairment associated with hippocampal dysfunction. The neuronal nitric oxide synthase/3',5'-cyclic guanosine monophosphate (nNOS/cGMP) pathway is implicated in synaptic plasticity, and in learning and memory processes. Here, we examined the nNOS/cGMP pathway in the hippocampus of HD mice to determine whether it can be...
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